"Samuel, only son of the above, was born in 1668. He was first of Hartford, then of Colchester, Conn., afterward of Bolton, Conn. He married Hepzibah Church, at Hatfield, Sept. 16, 1696. She was a daughter of Deacon Edward Church of Hatfield, Mass., son of Richard Church, one of the first settlers of Hartford. Mrs. Hepzibah Spencer died at Bolton, Sept. 13, 1745, in the 66th year of her age.—(Gravestone, Bolton burying-ground). Mr. Samuel Spencer died at Bolton, March 26, 1748, in the 80th year of his age.—(Gravestone, Bolton burying-ground)." - Hollister Family of America
Sources
Find A Grave: Memorial #58903026: Bolton Center Cemetery, Bolton, Tolland, Connecticut
Donald Lines Jacobus, The Four Spencer Brothers, The American Genealogist, V27 pp165-166
Paternal relationship is confirmed through Y-chromosome DNA testing. Frank Spencer (descendant of Thomas Spencer2) and Chet Spencer (descendant of Samuel3, William Spencer2), tenth cousins, match on 64 out of 67 markers (see YSearch IDs BVDU9 and 23V7R) thereby confirming their direct paternal lines back to their MRCA Gerard Spencer1
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DNA Connections
It may be possible to confirm family relationships with Samuel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree:
The 'confirmed witn DNA' indicator was reset. It can be set again once a DNA confirmation statement is included in the profile see Help:DNA_Confirmation for the details.
Please add a statement to this profile documenting the two matching yDNA tests used to confirm his relationship to his father, the number of markers the two test takers share, and the most recent common ancestor (MRCA) of the two. See this page for the format: https://www.wikitree.com/wiki/Help:DNA_Confirmation#Y-Chromosome_Confirmation
Thank you.
John Kingman, a Coordinator with the DNA Project