"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M4D2-GYX : 9 November 2014), Charles Cain in household of Amos Cain, Granville, Milwaukee, Wisconsin, United States; citing family 420, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
"United States Census, 1860", database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MW9W-4K6 : 30 December 2015), Charles B Cain in entry for Amos Cain, Wauwatosa, Milwaukee, Wisconsin, 1860.
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DNA Connections
It may be possible to confirm family relationships with Charles by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Charles: