A birth date (Chamberlain-818 born 28 Feb 1744) should not be within eight months of a sibling's birth date (Chamberlaine-15 born 17 Jul 1743) unless they are twins born within a day of each other.
The sister was Born 28 Feb 1744,
That is 7 1/2 months apart, so Margaret could have been born prematurely......
Biography
St. John's Parish Register
"Margarett Chamberlaine daughter of said persons was born Feb. the 23rd 1744." [1]
re Margaret's sister Mary married Thomas Lucas
> 2. REVEREND THOMAS3 LUCAS (THOMAS2, THOMAS1) was born 1733, and died
March 02, 1819. He married MARY CHAMBERLAINE March 02, 1762 in Baltimore City, Baltimore County, Maryland, daughter of JOHN CHAMBERLAINE and MARGARET GITTINGS.
She was born July 17, 1743 in Baltimore, Maryland.
Sources
WikiTree profile Chamberlain-818 created through the import of Gorham Family Tree.ged on Jan 27, 2012 by Dale Gorham. See the Changes page for the details of edits by Dale and others.
Source: S-2067278823 Repository: #R-2139932814 Title: Ancestry Family Trees Publication: Online publication - Provo, UT, USA: Ancestry.com. Original data: Family Tree files submitted by Ancestry members. Note: This information comes from 1 or more individual Ancestry Family Tree files. This source citation points you to a current version of those files. Note: The owners of these tree files may have removed or changed information since this source citation was created. Page: Ancestry Family Trees Note: Data: Text: http://trees.ancestry.com/pt/AMTCitationRedir.aspx?tid=15323412&pid=1066
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DNA Connections
It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her mitochondrial DNA.
Mitochondrial DNA test-takers in the direct maternal line: