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DNA Connections
It may be possible to confirm family relationships with Rowland by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Rowland:
The autobiography of John Chambers (later Governor of Iowa), son of Rowland Chambers and Phoebe Mullican states that his grandparents were James Chambers and Sarah Lee.
Rowland and Phoebe are my direct ancestors through my mother. I have researched them for years, and there is not a lot out there. The source referenced is one of the best I have found, although I am in the process of following up on some more promising ones. The Chambers lines are so screwed up by so many people online, that it is like navigating through a mine field! Probably a very good line for us all to collaborate on.. My line is through his daughter Catherine and her husband Joseph Kyle. He had another daughter married to Joseph's brother, so brothers married sisters. I do have Rowland's will somewhere, but it's very brief. Does mention Maxwell who has gone to North Carolina, and another daughter who married a Van Lear. I will try to find the info..buried right now due to a move.