Familysearch: United States Census 1850 shows Isaac, age 75, living in Brunswick Maine with Mary age 73, Sophia age 46 and Lucinda age 42 Clough
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DNA Connections
It may be possible to confirm family relationships with Isaac by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA test-takers in his direct paternal line.
Mitochondrial DNA test-takers in the direct maternal line: