"Maine Vital Records, 1670-1921," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:VZQF-X75 : 5 November 2017), Abial Holmes in entry for Ephraim Holmes, 25 Jun 1901; citing Machiasport, , Maine, United States, Division of Vital Statistics, State Board of Health, Augusta; FHL microfilm.
"United States Census, 1880," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MFQ4-CN5 : 15 August 2017), Ephraim Holmes, Machiasport, Washington, Maine, United States; citing enumeration district ED 185, sheet 329D, NARA microfilm publication T9 (Washington, D.C.: National Archives and Records Administration, n.d.), FHL microfilm 1,254,490.
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DNA Connections
It may be possible to confirm family relationships with Ephraim by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Ephraim: