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DNA Connections
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John:
top-right column, p 46, talks of John Jeffreys died 1718. Source given on profile shows this John's son was born 1716, so not ruled out by date, but nothing else really to connect them. Thought you might want to take a look though.