The 1851 census shows that his mother was previously married, probably to Samuel Whetcombe. Her surname at birth is unknown.
Sources
"England and Wales Census, 1851," database with images, FamilySearch (https://familysearch.org/pal:/MM9.1.1/SGGP-3G6 : 21 October 2017), Robert Knight in household of James Knight, Saint Mary Magdalen, Somerset, England; citing Saint Mary Magdalen, Somerset, England, p. 9, from "1851 England, Scotland and Wales census," database and images, findmypast (http://www.findmypast.com : n.d.); citing PRO HO 107, The National Archives of the UK, Kew, Surrey.
"England and Wales Census, 1871", database with images, FamilySearch (https://familysearch.org/pal:/MM9.1.1/VFJJ-NPX : 11 December 2017), Robert Knight in entry for James Knight, 1871.
"England and Wales Census, 1881," database with images, FamilySearch (https://familysearch.org/pal:/MM9.1.1/Q276-3TGR : 13 December 2017), Robert Knight, Taunton St Mary Magdalen, Somerset, England; from "1881 England, Scotland and Wales Census," database and images, findmypast (http://www.findmypast.com : n.d.); citing p. 43, Piece/Folio 2366/122, The National Archives, Kew, Surrey; FHL microfilm 101,774,716.
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DNA Connections
It may be possible to confirm family relationships with Robert by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Robert: