Eleanor's immigration indent record states her parents are "William Larkin, a labourer, native of Beckley and his wife Sarah Emsley";
Sources
NSW immigration indent record for "Ellen Milgate, 1838, the ship William Metcalfe";
Marriage record: England Marriages, 1538–1973 ," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:NK4H-9LW : 10 December 2014), William Larkin and Sarah Hemsley, 24 Apr 1791; citing Iden,Sussex,England, reference ; FHL microfilm 1,067,258.
1841 UK census: "England and Wales Census, 1841," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M7SN-1HJ : 30 October 2015), William Larkin, Beckley, Sussex, England; from "1841 England, Scotland and Wales census," database and images, findmypast (http://www.findmypast.com : n.d.); citing PRO HO 107, The National Archives, Kew, Surrey.
1851 census: "England and Wales Census, 1851," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:QKVN-Q8JG : 11 November 2015), William Larkin in household of James Ashenden, Beckley, Sussex, England; citing Beckley, Sussex, England, p. 13, from "1851 England, Scotland and Wales census," database and images, findmypast (http://www.findmypast.com : n.d.); citing PRO HO 107, The National Archives of the UK, Kew, Surrey.
1861 UK census: "England and Wales Census, 1861," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M7T9-JCY : 14 January 2016), Ann Ashenden in household of James Ashenden, Beckley, Sussex, England; from "1861 England, Scotland and Wales census," database and images, findmypast (http://www.findmypast.com : n.d.); citing PRO RG 9, The National Archives, Kew, Surrey.
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DNA Connections
It may be possible to confirm family relationships with William by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with William: