Paternal and maternal relationships are confirmed by an AncestryDNA test match between Annie's private granddaughter and the private daughter of Annie's brother Fielden Allen McCullough. Their MRCAs are their grandparents/great-grandparents, Thomas A. McCullough and Josephine Wilson. They share 296 cM across 17 segments. 1st cousin 1x removed probability: 43%.
"United States Census, 1900," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M36F-LZ3 : 20 January 2015), Annie Mccullough in household of Thomas Mccullough, Precinct 1, Vernon (incl. Vernon Town) & Precinct 3, Caryville & Precinct 11, Poplarhead Verno, Washington, Florida, United States; citing sheet 13A, family 234, NARA microfilm publication T623 (Washington, D.C.: National Archives and Records Administration, n.d.); FHL microfilm 1,240,177.
"United States Census, 1910," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MVK5-DZJ : 29 October 2015), Annie L Mc Cullough in household of Felen A Mc Cullough, Poplar Head, Washington, Florida, United States; citing enumeration district (ED) ED 118, sheet 10A, NARA microfilm publication T624 (Washington, D.C.: National Archives and Records Administration, n.d.); FHL microfilm 1,374,182.
"United States Census, 1920," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MNB6-TY4 : 14 December 2015), Annie Barker in household of C D Barker, Poplar Head, Washington, Florida, United States; citing sheet 3B, NARA microfilm publication T625 (Washington D.C.: National Archives and Records Administration, n.d.); FHL microfilm 1,820,232.
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DNA Connections
It may be possible to confirm family relationships with Annie by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Annie: