The witnesses to her marriage to Thomas Reeder were Thomas Whitton, Thomas Michael Harrison, Cassandra Blackburn and John Blackburn. Elizabeth gave her place of residence as Wakefield.
Sources
Listed as Francis Reeder's mother on Australia Death Index 1787-1985
Marriage Certificate, Thomas Reeder and Elizabeth Middleton (1847)
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DNA Connections
It may be possible to confirm family relationships with Elizabeth by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Elizabeth: