Isaac was born about 1780. He was a son of Benjamin Nichols and Comfort Beall.
He was lastly married in 1836, at about age 56, to Mary "Polly" Brewer in Pendleton County, Kentucky. It is not known if she had been previously married, but evident this was not Isaac's first marriage. He consented to the marriage of his daughter Nancy Jane Nichols to Hiram Race in Pendleton County on 19th July, 1840.
Sources
1836 Marriage of Isaac Nichols & Polly Brewer, Pendleton Co., KY
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DNA Connections
It may be possible to confirm family relationships with Isaac by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Isaac:
Nicholes-134 and Nichols-3667 appear to represent the same person because: These profiles represent the same Isaac Nichols of Grant Co. 1840-1860 who lastly married Polly Brewer in 1836.