John died on 14 Aug 1852 in Greene County, Indiana aged 45.
Sources
↑1850 Census:
"1850 United States Federal Census"
Year: 1850; Census Place: District 19, Sumner, Tennessee; Roll: 897; Page: 225a; Line Number: 1 Ancestry Sharing Link - Ancestry Record 8054 #6939292 (accessed 1 October 2022)
John Osburn (43), Farmer, in District 19, Sumner, Tennessee, USA. Born in Tennessee.
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DNA Connections
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John:
Got information from Father Bio https://www.findagrave.com/memorial/69314402/bartholomew-ozbun