Letitia was the daughter of John and Elizabeth (Patton) Preston. She married Col. Robert Breckenridge in July 1758. Letitia was Robert's second wife. Robert and his first wife had two sons. Letitia and Robert had 4 sons and one daughter:
William, m. Miss Gilham
John B., m. Mary Hopkins Cabell
James, m. Ann Selden
Elizabeth, m. Samuel Meredith
Preston, m. Miss Trigg
Research Notes
"He and Letitia had four sons and two daughters. John was twelve and James only nine when their father died in 1772."
Sources
Breckinridge Family Bible, Botetourt Museum, Fincastle Virginia
Blue Ridge Traditions 2004 Vol II, No3, p8, Part I: The Breckinridges by Malcolm W Bryan III.
The Preston genealogy; tracing the history of the family from about 1040, A.D., in Great Britain, in the New England states, and in Virginia, to the present time; by Preston, William Bowker. The Desert News, Salt Lake City, Utah, 1900. Pg 235 of 630
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DNA Connections
It may be possible to confirm family relationships with Letitia by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Letitia:
Preston-429 and Preston-698 appear to represent the same person because: same husband, birth date on -698 is uncertain with no source, other profile indicates birth is from marriage information