John's spouse was Mary/Polly Twombly, daughter Emily Sherburne was born in Sherbrooke,, Quebec, Canada in1807
Sources
Sandra De Blanc
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DNA Connections
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John: