Matilda G. Venable married Jackson Hopkins on September 7, 1859 in Johnson County, Tennessee. They had two children, James Hopkins and Benjamin Hopkins. Birth dates for the children aren't provided by the above internet site.
Sources
Jeff Martin
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DNA Connections
It may be possible to confirm family relationships with Matilda by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Matilda: