Denise, the GEDCOM you uploaded in March is still available for your use. If you choose to upload a new GEDCOM and create profiles from it, you can do so, but be advised you will have to start over again with the process of reviewing suggesting matches and marking them as matches or rejects.
You do not need to delete the old GEDCOM in order to upload the new one. It is possible to have more than two GEDCOMpare files at the same time, but be sure to give them distinctive names so you will not get confused.