The Maine Historical Magazine, Volume 6, 1891, p.35[1]
"Maine Births and Christenings, 1739-1900," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:F4WX-543 : accessed 1 February 2016), James Duning, 03 May 1786; citing BRUNSWICK,CUMBERLAND,MAINE; FHL microfilm 10,595.
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: