Ann (Kaylor) Hefner lived in Appalachia, in North Carolina. See Appalachia Project.
Sources
Ancestry family trees.
1850 U.S. Census, Catawba County, North Carolina, p. 320, born circa 1825; NARA microfilm publication M432, roll 624.
Find a Grave, database and images (https://www.findagrave.com/memorial/95061600/anne-caroline-hefner: accessed 19 May 2023), memorial page for Anne Caroline Kaylor Hefner (27 Aug 1825–15 Sep 1898), Find a Grave Memorial ID 95061600, citing Saint Peters Lutheran Church Cemetery, Conover, Catawba County, North Carolina, USA; Maintained by civilwarbuff (contributor 47049540).
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DNA Connections
It may be possible to confirm family relationships with Ann by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Ann: