Maternal relationship is confirmed by a triangulated group on GEDmatch who share a 24 cM segment on chromosome 5, consisting of F Lewis, GEDmatch kit # A616565, and RSR, her 5th cousin 1x removed, GEDmatch kit # A311258, and SB, her 5th cousin 1x removed, GEDmatch kit # A926466. (RSR and SB are 6th cousins.) Their most-recent common ancestors are Thomas Bastin and Hannah Brockman, the 4x great grandparents of F Lewis and 5x great grandparents of both RSR and SB.
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DNA Connections
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John: