Ebenezer's will was probated on May 4th, 1790 so he passed away before this date.
Sources
Baldwin Thomas W., complier, Et al., Vital Records of Mendon Massachusetts: to the Year 1850 (Boston, Mass: Wright & Potter Printing Company, 1920), page 126.
"Massachusetts, Town Clerk, Vital and Town Records, 1626-2001," database with images, FamilySearch (13 July 2016), Worcester > Mendon > Births, marriages, deaths 1643-1835 > image 134 of 428; town clerk offices, Massachusetts.
Charles Henry Pope, Merriam Genealogy in England and America: Including the "Genealogical Memorand" of Charles Pierce Merriam, the Collections of James Sheldon Merriam, etc. (Boston, Mass.: Charles H. Pope, 1906), page 78.
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DNA Connections
It may be possible to confirm family relationships with Ebenezer by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Ebenezer: