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DNA Connections
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John:
Source: The Original Scots Colonists of Early America. Supplement 1607-1707 Surnames, O-P https://www.ancestry.com/interactive/48521/ScotsColonistsAmSupp-001105-137/378468?backurl=http%3a%2f%2fsearch.ancestry.com%2fcgi-bin%2fsse.dll%3fgst%3d-6&ssrc=&backlabel=ReturnSearchResults&rc=954,212,1121,243;90,1117,309,1146;91,1212,317,1242;91,1399,320,1429