"David (son of Daniel), in his will dated 2nd day of January, A. D. 1776,
probated in Phila., August 18th, 1776, names legatees his widow, Barbara, and children-John, Mary, Magdalena, David, Jacob, Henry, Daniel, Peter, and Isaac, the last five being minors;" 
It may be possible to confirm family relationships with Barbara by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Barbara:
Deb Love :
AncestryDNA, GEDmatch A110529, Ancestry member deblove85 +
Autosomal DNA Test, GEDmatch z267505