Clifton Alley's DNA has been tested for genealogical purposes. It may be possible to confirm family relationships by comparing test results with Clifton or other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree:
Family Tree DNA Y-DNA Test 67 markers, haplogroup R-M269, FTDNA kit #131933
Mitochondrial DNA test-takers in the direct maternal line: