Mary was born about 1849. She was the daughter of Charles Alwine and Mary Timble. She passed away in 1882.
Sources
"Philadelphia, Pennsylvania, U.S., Death Certificates Index, 1803-1915," database with transcriptions, Ancestry (https://ancestry.com : downloaded 2 December 2020), transcribed death index, Mary Emma Alwine, 17 June 1882; citing ""Pennsylvania, Philadelphia City Death Certificates, 1803–1915.""
"United States Census, 1850," database with images, Ancestry (https://ancestry.com : downloaded 2 December 2020), image census page, Mary C Olwine, 12 August 1850; citing "Seventh Census of the United States, 1850; (National Archives Microfilm Publication M432, 1009 rolls); Records of the Bureau of the Census, Record Group 29; National Archives, Washington, D.C."
"United States Census, 1860," database with images, Ancestry (https://ancestry.com : downloaded 2 December 2020), image census page, May Alwine, 20 June 1860; citing "1860 U.S. census, population schedule. NARA microfilm publication M653, 1,438 rolls. Washington, D.C."
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DNA Connections
It may be possible to confirm family relationships with Mary by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Mary: