"United States Census, 1940," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:VR14-RNJ : 30 January 2020), Betty L Shacklett in household of Annie M Andrews, Strasburg, Davis Magisterial District, Shenandoah, Virginia, United States; citing enumeration district (ED) 86-6, sheet 7B, line 49, family 144, Sixteenth Census of the United States, 1940, NARA digital publication T627. Records of the Bureau of the Census, 1790 - 2007, RG 29. Washington, D.C.: National Archives and Records Administration, 2012, roll 4294.
"Virginia, Marriage Certificates, 1936-1988," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:QK9N-QG5R : 8 April 2020), Harry Lee Neff and Betty Lee Shacklett, 08 Apr 1950; from "Virginia, Marriage Records, 1700-1850," database and images, Ancestry (http://www.ancestry.com : 2012); citing , Shenandoa, Virginia, United States, certificate 9505, Virginia Department of Health, Richmond.
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DNA Connections
It may be possible to confirm family relationships with Gladys by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Gladys: