Levi Ashbrook was the son of Aaron Ashbrook and Hannah Ward Cook. He is buried in the Ashbrook Plains Burying Ground, Romney, Hampshire Co, VA (now West Virginia). Levi last lived in what is now Berkeley Co, WV. He was married (1st) to Mary Pentecost. It is believed that she is buried in Romney, WV. He was married (2nd) to Mary Chenoweth who was the first known daughter and seventh child of John and Mary Smith Chenoweth. She married at the age of 20, Levi Ashbrook, ten years her senior. Levi, a preacher and Justice of the Peace, was a widower with three or four children. He would later serve in the Revolutionary War. Together he and Mary would produce 12 more children, all probably born in Hampshire County. This next generation would extend the Ashbrook-Chenoweth ties. Mary's stepson, Levi, and two of her own sons, John and Absolum, married Chenoweth daughters of Mary's brother, John Chenoweth and probably his second wife Eleanor Parke. These latter two of were first cousin marriages. Another four Ashbrook children would marry into the Peters family. The Ashbrook lines would spread to Kentucky, Ohio, Indiana and Illinois. Levi died in his fifties in 1794 and Mary remarried ten years later to James McBride of Virginia, who she also survived. She went to Ohio and is buried at Turkey Run in Fairfield Co, OH. Levi Ashbrook was a veteran of the Revolutionary War having served as a Lieutenant. He was a Baptist Minister. Levi Ashbrook was census enumerator in his district in Hampshire Co, VA in 1782 and 1784. He served on a jury in the same county in 1782. Preacher Elder Levi Ashbrook is recorded in the Baptist Association Library at Chester, PA, as a preacher in North River Church near Romney, VA from 1790 to 1793. It is stated they thought illness and death ended his charge there. 
Source: S-1320841970 Repository: #R-1587562506 Title: A roster of Revolutionary ancestors of the Indiana Daughters of the American Revolution : commemoration of the United States of Author: Ancestry.com Publication: Online publication - Provo, UT: Ancestry.com Operations Inc, 2005.Original data - A roster of Revolutionary ancestors of the Indiana Daughters of the American Revolution : commemoration of the United States of America bicentennial, July 4, 1976. Evansville Note: APID: 1,29740::0
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It may be possible to confirm family relationships with Levi by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Levi: