Harriett Atheral was born before 24 Apr 1842 as she was baptised on that date at Withyham, Sussex.  Her parents were John Atherall and Frances "Fanny" (Card) Atherall
Harriett married John Pilcher (there is no evidence that he was called Ruben) on 21 Aug 1861 in Withyham, Sussex. 
John and Harriet had at least 2 children
Alice in 1863 and Albert in 1866
Harriett passed away aged 63 in Q4 1901 in Wandsworth, London. 
↑Harriett Atheral Baptism:
"England & Wales, Christening Index, 1530-1980" Ancestry Record 1351 #1665233 (accessed 14 May 2022)
Harriet Atherall baptism on 24 Apr 1842, daughter of John & Fanny, in Withyham, Sussex, England.
"England Marriages, 1538–1973"
FamilySearch Record: N6KP-JPS (accessed 14 May 2022)
John Pilcher marriage to Harriet Atherall on 21 Aug 1861 in Withyham, Sussex, England, United Kingdom.
↑Harriett Pilcher Death Registration:
"England & Wales General Register Office" GRO Online Indexes - Death (accessed 14 May 2022)
Pilcher, Harriett (Age at death: 63). GRO Reference: 1901 Oct-Nov-Dec in Wandsworth Volume 01D Page 358.
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It may be possible to confirm family relationships with Harriett by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Harriett: