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Barbe Bajolet is one of the founding mothers of Acadia. She was baptized on June 22 1608 at Piney in ancient Champagne province, in present date France's Aube department.  She was the daughter of Antoine Bajolet and Jeanne Baudinet.
Around 1629 she married Isaac Pesseley in France. Between about 1630 and 1645, the couple had eight children: Etienne, Marguerite, Perrette, Jeanne, Charlotte, Henriette, Gilles, and Marie. The first three children Etienne, Marguerite and Perrette were baptised at Piney (Champagne) France. Stephen White provides no specific birth dates or locations for the remaining children born between about 1638 and about 1645.
On April 1, 1636, Isaac sailed on the Saint-Jehan towards Acadia. He appeared on the ship's roster: "Isaac Pesselin de Champagne".  However, unlike some others listed, no wife or children are on the passenger list. Therefore, it is not known exactly when Barbe arrived in Acadia.
She returned to France some time before or after her husband Isaac was killed in 1645 because she married again at St-Barthélemy de La Rochelle, France in 1646. The marriage contract (contract of Cherbonnier dated 16 Dec 1646) between Barbe and her spouse Martin Lefebvre de Montespy mentions that she had just returned from Canada. The religious ceremony took place on January 10 1647. They had two children, both baptised at St-Jean de La Rochelle: Louise (b. 29 Oct 1647) and Marie-Anne (born. 10 Oct 1649; baptised 18 april 1650).
Barbe was still in La Rochelle when her husband Martin passed away in 1651 in that city. 
Her third marriage took place on October 17 1654, at St-Jean de La Rochelle, Aunis, France. Her husband was Savinien de Courpon, widower of Olive de Laubaure. No children were recorded from this marriage.
Barbe returned to Acadia with her third husband in 1654.  They settled in Cap Sable on a fief conceded to them by Charles de la Tour. After Savinien passed away in 1661, Barbe and her children left Cap Sable for Port-Royal.
The 1671 census lists widowed Barbe, 63 years old, living in Port-Royal, the owner of 1 cow, 5 sheep but without land.
The 1678 Port-Royal census shows Barbe, listed as widow Pesselet, living in the household of Francois Levron and Catherine Savoye. 
Barbe Bajolet passed away after 1678 in Acadia.
The Mothers of Acadia Maternal mtDNA Project is conducting ongoing research to verify their origins. In 2010, Stephen White reported Barbe Bajolet had an X2b haplogroup. Details of how many of Barbe Bajolet's descendants were tested in White's report are not known. Lucie Leblanc Constantino reported one result here, interpreting the result as of European origin. Ongoing test results are also reported [ here]. As of July 2016, 7 descendants have reported a haplogroup X, of which three have a full sequence test, refining the haplogroup to X2b7. Most of the time, X haplogroup indicates European origins. Acadian haplogroup X belongs in Native American haplogroup X when mutations 8913G, 12397G, and 14502C are present. To date, these mutations are not found in the reported results.
Based on the ADN Héritage Française / French Heritage DNA project, the Catalogue de Signatures ancestrales validées par ADN website documents mtDNA triangulation (reference TRI0140) for Barbe Bajolet to be Haplogroup(e) ADN-mt X2b7. TRI0140 traces three living individuals through the mothers back to the mother of Barbe Bajolet born in France.
Maternal relationship is confirmed by an exact HVR1 and HVR2 match between this Family Tree DNA mtDNA test for Donna (Friebel) Storz and this Family Tree DNA mtDNA test of her maternal line cousin Mary-Lucille (Andrus) Sims.
Barbe Bajolet's baptism date
Every reference cited indicates that she was baptized on May 22, 1608. However, examination of the actual parish register shows that the baptism took place in June. In response to an e-mail inquiry, Stephen A White concurs that Barbe Bajolet's baptism date should be changed from May to June.
Barbe Bajolet, fille de Antoine, premier muletier de la reine Marie de Médicis, et Jeanne Baudinet, fut baptisée le 22 juin 1608 à Piney dans l'ancienne province française de Champagne, auj. dans le département de l'Aube. Antoine Contant était le parrain et les marraines furent Barbe, épouse de Pierre Pesseley et Jeanne, veuve de Jean Pesseley.
Son premier mariage, vers 1629, était avec Isaac Pesseley. Elle se maria de nouveau à St-Barthélemy de La Rochelle le 10 janvier 1647 avec Martin Lefebvre de Montespy. Son troisième mariage eu lieu le 17 octobre, 1654, à St-Jean de La Rochelle, Aunis où elle prit Savinien de Courpon comme mari. Savinien était veuf d'Olive de Laubaure.
Barbe BAIOLET, widow of Saviniue de COURPON; Children: 8 children in France and 2 daughters married in this country (Marie PESELET 26, Marianne LEFEBVRE 21); cattle 1, sheep 5.
The Widow Pesselet, Francois Levron & Catherine Savoye, 1 boy Jacques born 1677. [sic, this woman, was married to two other men since PESSELET.]
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