Autosomal DNA: Close Relationship. Susan Barley's great-granddaughter (GEDmatch.com ID A257419) shares 18 cM with a descendant (GEDmatch.com ID A846024) of Stephen Barley and Nancy of the 1850 census in Marengo County, Alabama. The segment has not been triangulated; however, A257419 and her grand-nephew and grand-niece have other AncestryDNA matches to descendants of Stephen Barley (who are not using GEDmatch).
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It may be possible to confirm family relationships with Susan by comparing test results with other carriers of her mitochondrial DNA.
Mitochondrial DNA test-takers in the direct maternal line: