Thomas Barnes-217 which may be the lowest number Thomas of Hartford with correct spouses and children attached. See Barnes-217.
Thomas Barnes "of Hartford" was previously attached as the father of this Thomas Barnes. There is nothing to substantiate this claim. Nothing has been found about the parents of this Thomas Barnes, and Thomas Barnes of Hartford apparently didn't have a son Thomas, so he has been detached as the father.
THOMAS BARNES, referred to as " Thomas of New Haven and Middletown," was in New Haven in 1644. He married 1st to Mary _____. She died Apr 1676, and Thomas married 2nd to Elizabeth _____ . Thomas had a brother Daniel. Thomas died 10 June 1691 in Middletown, Connecticut. Elizabeth died 1694 according to Jacobus, but 4 Jan 1689/90 according to the Barbour Collection of Vital Records.
Life in New Haven
“Tho: Barnes” took the Oath of Fidelity 1 July 1644.
He and his wife had seats in the meeting house.
New Haven fined a lot of its citizens. Thomas was not an exception. He was fined twice for not having a ladder, in 1643, and 1649. The ladder was for the purpose of putting out chimney fires. In 1650, some of his fence was broken and horses came in and he was fined.
17 May 1647, Thomas was chosen as fence viewer “For Mr. Grigsons quarter, Henry Lindoll and Thomas Barnes, but Thom Welch is to satisfie Thom Barnes for his paynes, or els to pvide another.
Thomas also testified in court, helped take estate inventories, and Sept 23 1667, Thomas Barnes & others were “defired & appointed to view the land on both fides of ye eaft River againft playnes & make report to ye towne how they find it in reference to a village.”
Thomas acquired land by town grant and by purchase from others. 
6 May 1645 “Thomas Barnes required satisfactio of Raiph Dighton for a cow of his wch perished as he conceives through his sons neglect who kept the heard thatt day, but Raiph Dighton alleadged thatt a cow of thatt heard being swamped, his son came home to the towne to get help, and left his partner wth the cow in the interim, and before they had gott the cow out of the swamp itt was night and the heard was coming home and they nott knowing of any dangerous place betwixt the bridge and the place where the cattell vsed to come over, did not conceive itt necessary to follow the cattell, but came over the bridge, and though they came on the other side of the river over against the place where his cow was afterwards found perished wth her foote in a hole betwixt the banck and the roote of the tree, yet they did neither heare nor see her. [others also said there was no known place]. The judgmt of the Court was thatt itt was an afflicting providence of God wch the said Barnes was to beare himselfe, and that the boy was innocent in the case.
5 Dec 1648. Thomas Barnes served a warrant on James Till who had taken his scythe. Till was fined and punished for theft, contempt of court and slandering Thomas Barnes.
Mercy marr 1st 20 Dec 1666 at Branford , CT to Bartholomew JACOBS, marr 2nd 22 Nov 1694 to Joseph THOMPSON
John baptized Nov 1694 (adult) died 1712 marr 16 Nov 1669 to Mercy BETTS daug of Roger BETTS
Elizabeth b 28 May 1650 marr 1st 24 Mar 1668/69 to Benjamin BROCKETT.... marr 2nd 21 Jan 1684 John AUSTIN
Thomas b 26 Aug 1653 d 1712 marr 1st 26 June 1675 May HUBBARD marr 2nd 1690 Abigail FROST, daug of John & Mercy (Payne) Frost
Abigail b 11 Mar 1656/7 [see her profile for discussion of marriage]
Daniel b 29 Aug 1659 d 1 Jan 1730/1 marr 13 Jan 1686 to Mary TAPPEN
Maybee b 25 June 1663 d 6 Mar 1749 marr 19 Nov 1691 Elizabeth STOW, daug Rev Samuel & Hope (Fletcher) Stow
Will dated 25 Feb 1683/4 mentions "my wife, my sons John, Thomas, Daniel, Maibe ... daug Mercy Jacobs, Martha, Elizabeth, Abigail, witness validated 6 Oct 1692, accepted and recorded 7 Sept 1693 
The Last Will and Testament of Thomas barnes Senior dwelling in Midleton, in the County of Hartford and Colony of Connecticutt is as followeth. I being at present under considerable bodily weakness, yet having the use of my understanding as formerly, yet not knowing the day of my death, and being willing to leave peace behind me among my children when I shall be taken from them, do leave this as my last will and testament after committing my Spirit to god which gave it and my body to a descent burial, I do dispose of that portion of worl[dl]y goods which God hath given me as followeth.
IMPRIMES I give to my eldest son, John Barnes, fourty acres of upland which I bought of Mr. Mose and Thomas Wheller, lying together, moreover I give to my said son that land on which his house standeth, bought of old Goodman Wheeler, to run from the front line to the rear of the lots, and I Give him also a part of that land I bought of old Goodman Loe, from the front to the common road; moreover, I give to my said son a parcel of meadow beginning at the head of the two Coves and so to run west to the branch that comes out of the great pond, to Brown's line and six rods beyond that branch.
IT I give to my son Thomas Barnes that parcel of upland where he hath built his hous, to lie on the south sid from the crooked tree where Mr. Bracet and I parted, to the Great Chestnut tree and so by the bogie meadow side till they com at the old lines; moreover I give him halfe my shrubby plain lott; I give him a piece of land I bought of Loe and Els (Ellis?); that is from the front line to the meadow; the one half of that I give to Thomas and the other half to my son Danile; more I give to my said son Thomas the eastward side of the great meadow according as(?) he and Daniel have divided it; as also a piece next my son John's meadow, beginning where John endeth and soe running westward till he come within forty rods of the river, all these lands butted as they are already laid out and all that is between the front stake of my son John's next Eliazer Brown till it comes to Thomas More's northward from it, butting on the meadow westward.
IT I give to my son Daniel Barnes all that upland on the north side of my son Thomas is as far as my land goeth and half my lot on the shrube plain and of that I bought of Andrew Loe; I give him that part from the common road to the rear of that lot and that half mentioned by Thomas; moreover I give him, my said son, the westward side of the great meadow as Thomas and he have devided with that piece below, next Thomas, all butting on Brown's line abutting on the river westward.
IT I give to my daughter, Mercy Jacobs, twenty acres of upland, that is that land on which their house standeth; that is to her and her youngest son, to them and their heirs forever; moreover I give to her twenty shillings to be paid her by my son John after my decease.
IT I give to my daughter Martha, twenty shillings, my son John to pay her it after my deceas.
IT I give to my daughter Elizabeth, twenty shillings, my son Daniel to pay it her after my deceas.
IT I give to my youngest son maibe Barns all my Land in Midleton after my wivs deseas all but the hous and home lott at my wif Deceas the hous and home lott I will to my loving wife During her naturall Life more over I Give my said wife two Cows and my will is that my son maibe shall look after and provid for the keeping thos two cows which I give my wife my will is my wife shall have all my movables during her naturall life.
IT I Give to my daughter abigaill three pounds and my Son maibe to pay it her after my deceas
I give allso to my loving wife all my bees and Sheep if there be any remaining my will is that after my wives deseas all my Utensells belonging to the hous shall be my son maibee and what Cattell shall be remaining after my Deceas I give to my said son maibe farther my will is that as I have Done acording to my ability for my sons John and Thomas and Danill too I expect and it is my will that John and Thomas shall pay to their mother of five bushells of wheat a year yearly During the tim of her widowhood and Danill five bushels of wheat yearly During the same time.
↑ Connecticut County, District and Probate Courts. Middletown 1692, #345-a Hartford Probate District. Thomas Barnes.
Probate Packets, Barker, Ethan-Barrows, B, 1641-1880. at Ancestry.com. Connecticut, Wills and Probate Records, 1609-1999
It may be possible to confirm family relationships with Thomas by comparing test results with other
carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Thomas:
Barnes-11699 and Barnes-775 do not represent the same person because: This Thomas Barnes (Barnes-11699) has the birth and death dates for Thomas Barnes-775 (birth 26 Aug 1623 and death 10 Jun 1691), but the daughter attached to him (Sarah Barnes-11698 whose married name is Scoville) belongs to Thomas Barnes of Hartford and Farmington (Barnes-217). [Note that Sarah Barnes-11698 is a duplicate of Sarah Barnes-187.] I'm rejecting the merge of Barnes-11699 and Barnes-775 and will propose a merge of Barnes-11699 with Barnes-217.
I agree with Theodore Palmer. These do not look to me like the same Thomas Barnes. There are different dates for both their birth year and for death year. Each Thomas was married to a Mary Unknown, but the information for the two Mary Unknowns is also quite different. Barnes-2267's wife Mary died in April 1676 according to Jacobus. But Barnes-3924's wife Mary died in 1661. And there are children attached to Barnes-3924's wife: Sarah, Joseph, Benjamin, and Hannah, who do not belong to Thomas Barnes-2267. I have changed this to a Rejected Match.
Barnes-3924 and Barnes-2267 appear to represent the same person because: There are many people named Thomas Barnes, but both of these profiles are for the father of Daniel b.1659. If there are any conflicts between these two profiles, please use the information in profile Barnes-2267. Thanks.