Elizabeth Bartley (last name may have been Bartlett or Bartell) began as a hired girl in the Mansker house. Samuel had children with Elizabeth while he was still married to Nancy Crawford. After Nancy died, Samuel married Elizabeth, the second of Samuel's four wives.
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It may be possible to confirm family relationships with Elizabeth by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Elizabeth: