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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James:
I have Polly Andrews as his mother- This is from Find a grave :URLhttps://www.findagrave.com/memorial/30797823. I also have Nancy Hunter Coles as his wife(not sure if one and the same Nancy or not}