Thomas Berkeley, 5th Lord Berkeley was born in 1472 at Thornbury, Gloucestershire, England. He was the son of Sir Maurice Berkeley and Isabel Meade. He married, firstly, Eleanor Constable, daughter of Sir Marmaduke Constable and Joyce Stafford, between 1504 and 1505. He married, secondly, Cicely Arnold in 1526.
He also went by the nick-name of Thomas 'the Sheep Master'. He was invested as a Knight on 9 September 1513 from the Earl of Surrey He fought in the Battle of Flodden on 9 September 1513. He held the office of Constable of Berkeley Castle on 15 May 1514. He held the office of Sheriff of Gloucestershire between November 1522 and November 1523. He succeeded to the title of 5th Lord Berkeley [E., 1421] on 12 September 1523, by writ.
Thomas Berkeley had his will drawn up on 11 Jan 1533, describing himself as Thomas Berkeley, Knight, Lord of Berkeley.  He made bequests to and named:
He died on 22 January 1532/33 at Mangotsfield, Bristol, Gloucestershire, England. He was buried at St. Augustine's, Bristol, Gloucestershire, England. His will (dated 28 April 1532 to 11 January 1532/3) was probated on 6 June 1533
In his will he made particular note of the burial rites that he wanted; firstly to be buried in the church at Mangotsfield, Gloucestershire between the choir and his chapel; and then, a year after his death, his body to be exhumed and taken to the monastery of St Augustine at Bristol, to be reburied beside his first wife Eleanor.
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