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William Brooke's DNA has been tested for genealogical purposes. It may be possible to confirm family relationships by comparing test results with William or other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree:
Family Tree DNA Y-DNA Test, haplogroup R1b1a2, Ysearch YUQ5G, FTDNA kit #101038
It is likely that these autosomal DNA test-takers will share DNA with William: