James Bush's DNA has been tested for genealogical purposes. It may be possible to confirm family relationships by comparing test results with James or other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree:
Family Tree DNA Y-DNA Test 37 markers, haplogroup R-M269, FTDNA kit #289092
Mitochondrial DNA test-takers in the direct maternal line:
Family Tree DNA mtDNA Test Full Sequence, haplogroup V, FTDNA kit #289092
It is likely that these autosomal DNA test-takers will share DNA with James:
Family Tree DNA Family Finder, GEDmatch T122823, FTDNA kit #289092