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It may be possible to confirm family relationships by comparing test results with R or other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree:
Family Tree DNA Y-DNA Test 37 markers, haplogroup R-M269, FTDNA kit #399115
It is likely that these autosomal DNA test-takers will share DNA with R: