Father Sir Robert Constable, Sheriff of Yorkshire, Justice of the Peace for Yorkshire b. b 1495, d. 29 Oct 1558
Mother Katherine Manners b. c 1511
Barbara was born about 1531. Barbara was the child of Robert Constable and Katherine Manners.
Barbara was the first wife of Sir William Babthorpe. Suspected of Catholicism, Babthorpe proved his loyalty to Elizabeth I by helping to quell the ‘Northern Rebellion’ of 1569 which attempted to place Mary Queen of Scots on the throne.
Among the couple’s children was Margaret Babthorpe. Barbara passed away before 1558. 
Sir William Babthorpe b. c 1530, d. 1 May 1581
Margaret Babthorpe, wife of Sir Henry Cholmondley 
It may be possible to confirm family relationships with Barbara by comparing test results with other carriers of her mitochondrial DNA.
Mitochondrial DNA test-takers in the direct maternal line: