Paternal and maternal relationships are confirmed by an AncestryDNA test match between Annie's private grandson and Annie's private sister. Their MRCAs are their parents/great-grandparents, Loran Cook and Oneta Barber. They share 607 cM across 23 segments. Grandaunt/grandnephew relationship probability: 25%.
"United States Census, 1940," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:VTZ3-CJM : accessed 7 October 2015), Annie L Cook in household of Loran L Cook, Election Precinct 14, Washington, Florida, United States; citing enumeration district (ED) 67-18, sheet 7B, family 132, NARA digital publication T627 (Washington, D.C.: National Archives and Records Administration, 2012), roll 622.
"Florida Divorce Index, 1927-2001," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:VK7R-747 : 25 December 2014), William W Watford and Annie Lou Watford, Dec 1964; from "Florida Divorce Index, 1927-2001," database and images, Ancestry (http://www.ancestry.com : 2005); citing Escambia, Florida, certificate 22615, volume 1107, Florida Department of Health, Jacksonville.
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DNA Connections
It may be possible to confirm family relationships with Annie by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Annie: