[This profile represents two different men named John Crawford and must be separated into different profiles by the profile managers and others at this point.] - Not proven to be the same John Crawford except by association on WikiTree.
Note on DNA for this John Crawford: The place of Crawfurd/Crawford Scotland is near the western Border Marches along the Scots - English border. Most men from this area test R1b haplogroup, not I2 which associated with the Viking invasion/immigration/incursion into the NE English kingdom called Mercia, later Northumberland (Durham, York) England. This doesn't negate the possibility that a John or his ancestor may have migrated from Crawfurd/Crawford Scotland originally, but the surname may well be more of a geographic reference than a true lineal surname.
Research Note - Important! please read
Based upon a preponderance of the evidence we appear to have mixed two different John Crawford's in this profile. Maybe both were "Ensign" but who knows until primary records are produced.
Essentially, a John Crawford from Hanover county VA who married Mary Duke before migrating down to early NC to survey lines and that had well-documented children with consistent middle names cannot be the same John Crawford married to an Elizabeth with 3 differently-named children (no mention of Mary Duke children) in a will and deeds from a now-extinct Craven county SC (now multiple coastal SC counties). Not sure how the mix-up originated but these are two different people by geography, spouse and children. The children listed as John, Michael or Thomas in Craven county SC are not proof of the John Crawford of Hanover county VA except by timing and loose association (per the sources identified).
John was born in 1701. He is the son of Captain David Crawford (1662-1762) and Elizabeth Smith.
John Crawford, formerly of Hanover County (VA), entered the Carolina colonies in an area which would later become Anson and Richmond County (NC) as an occasional attorney and surveyor around 1737.
Note that this will only names three children.
Will of John Crawford of Craven County in the Province of South Carolina. Charleston Co., SC, Record of Willis, 1760-1767, pp. 52-53, written 20 Oct 1756, proved 10 Jan 1761. Digital image at Ancestry.com - https://preview.tinyurl.com/y5uuoms5
wife Mary Crawford
son John Crawford - land where he lives on the lower side of Phill’s Creek
son Thomas Crawford- remainder of land on Phill’s Creek; land adjoining Abraham Lundy
son Michael Crawford - 300 acres, manor house and plantation, land on Naked Creek containing 750 acres
Wants land on Lynch’s Creek to be sold.
300 pounds in Virginia (tobacco? money?)
Exrs: wife Mary, sons John & Thomas
Wit: Charles Thomson, Durham Hills, Archd McNorton
On 29 Apr 1767, Thomas Crawford of St. Mark's Parish, Craven Co., SC, sold his land on Phill's Creek to Thomas Lide. 
On 22 Sep 1767, Michael Crawford sold his 300 acres to his brother, Thomas Crawford. On 1 Oct 1767, Thomas Crawford sold this land to Charles Bedingfield, both of Craven Co., SC. 
1. It appears that this material may relate to another John Crawford from Pennsylvania (the James Crawford mentioned is James Crawford (1701-1749), his son John was not born until 1733, unknown as of yet who John Crawford is referring to here)
3. Source S153
He passed away before 1761.
"South Carolina, 29 Apr 1767. Book G-3, p. 579. L&R. Thomas Crawford, planter & Elizabeth his wife to Thomas Lide, planter, both of St. Marks Parish, Craven Co., for £1500. 150 a. on SW side Peedee River, granted 24 Mar. 1756 by Gov. James Glen to Abraham Lundy; who conveyed to John Crawford; who bequeathed to his son, said Thomas; bounding SW on James Gillespie & vacant land; NW on William Hainsworth. Wit: John Winters, John Bohannon. Bef. Thomas Wade, JP. Rec. 7 Aug 1767."
"South Carolina, 1 Oct 1767. Book L-3, p. 401. Conveyance. Thomas Crawford, planter, to Charles Bedingfield, tavern keeper, both of Craven Co., for £1500, 300 a. known as Carraw Bluff on NE side of Pee Dee River on which Michael Crawford now lives"..."which tract was granted 29 Jan 1742 by Lt. Gov. William Bull to David Roach who by L&R dated 10 & 11 July 1749 to John Crawford Sr.; who bequeathed to his son Michael Crawford; who on 22 Sept. 1767 conveyed to Thomas Crawford. Wit: John Bohannon, Elizabeth Bohannon. Before Alexander Mackintosh, JP, on 26 Sept 1768. Rec. 10 Oct 1768."
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