John Pickup was married 1. Hannah Lord, had a family. His son John William married Isobells sister Margaret Crichton. Some of the descendants of John and Hannah didn't know john married again and had a second family.
Isabella was born in 1854. She passed away in 1929.
Sources
Family knowledge, birth, marriage death certs, news paper articles from England, census records
Census, records from Scotland
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DNA Connections
It may be possible to confirm family relationships with Isobell by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Isobell: