James Dixon and Ann Killian eloped. He was ethnic English and Protestant. She was Irish and Catholic. Both families disowned them totally. Margaret was the oldest child. James died when some of his children were still young.
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It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: