Parents cannot be William Douglas and
Isabel Elizabeth Kennedy. It is known that William, who was the 4th Lord of Mouswald, died without issue, as the title passed on to one of his brothers, John, who became 5th Mouswald.The family line of the Douglases of Mouswald would later become extinct.
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It may be possible to confirm family relationships with William by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: