Ann Downing is a signatory of the 1811 deed mentioned in the profile of her father John Downing. Prof. Beckham indicates that she was born in about 1769 and died in about 1843. See her father's profile.
Never married
Sources
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DNA Connections
It may be possible to confirm family relationships with Ann by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Ann: