He was born in Mirow to Charles Louis Frederick of Mecklenburg and his wife Princess Elizabeth Albertine of Saxe-Hildburghausen. His father was the second son of Adolphus Frederick II, Duke of Mecklenburg.
It may be possible to confirm family relationships with Adolphus Frederick IV by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Adolphus Frederick IV: