His children were:
John H., Mary A., Catherine A., Jacob F., Samuel F., Susan M., Peter A., Lydia A., Emmy Jane, Hannah Amanda, Abraham L., Ira, Clinton, and David Jacob Eisenhower
Paxson Link, pg 180: ".....Reverend Jacob Eisenhower, a 'Pennsylvania German' (member of the sect known as river Brethren...." pg 181: "....Jacob's standard gift to his children, upon their marriage, was a one-hundred-sixty-acre farm and two thousand dollars in cash......." page 374: "......Reverend Jacob Eisenhower, an ordained pastor of the River Brethren, a Mennonite sect........"
The Link Family, Antecedents and Descendants of John Jacob Link, 1417-1951," Paxson Link, l951, page 180,181, 174.
Lane, Michelle, Re: (Margaret)Rebecca Matter, Eisenhower - message on Matter Genealogy Forum - 11 Apr 1999.
Roberts, Gary Boyd., Christopher Challender Child, and Julie Helen. Otto. Ancestors of American Presidents. Boston, MA: New England Historic Genealogical Society, 2009. Print.
"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M4HH-NKL : 9 November 2014), Jacob Eisenhower, Lower Paxton, Dauphin, Pennsylvania, United States; citing family 49, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
1860 U.S. Census - Elizabethville, Washington Twp., Dauphin Co., PA - Roll# M653_1104; p726; Image# 121; FHL# 805104 .
"United States Census, 1870," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MZGK-4N7 : 17 October 2014), Jacob Eisenhower, Pennsylvania, United States; citing p. 18, family 120, NARA microfilm publication M593 (Washington D.C.: National Archives and Records Administration, n.d.); FHL microfilm 552,834.
Cramer, Roger - e-mail to George G. Stradtman, Jr. - 6 Sep 2014.
"Descendants of Hans Matter" report forwarded to George G. Stradtman, Jr. by Roger Cramer on 6 Sep 2014 as e-mail attachment .
SAR Membership App. - Vol.# 328; SAR Membership# 65450 .
Londeen, Donna Eldorf - "Jacob Frederick Eisenhower" FindAGrave Mem.# 34561411 .
It may be possible to confirm family relationships with Jacob by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: