It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with James:
Petition for a new county out of Nansemond and Isle of Wight in 1784 (which didn't happen)...
http://www.therainwatercollection.com/reference/ref123.pdf has quite a few names interesting to this family, including James' own and his brother's. It seems there may have been a number of petitioners who, having failed to form a county, moved (or completed a move already under way) south into North Carolina shortly after.