Jonathan studied medicine with his brother. While visiting a patient, he was thrown from his horse and injured, from the effects of which he died. He was not married.
Sources
History of Clinton County, Ohio : containing a history of the county, its townships, cities, towns, schools, churches, etc.... ,...Published in 1882. Page 928-9. [1]
Genealogy of the Sharpless family, descended from John and Jane Sharples, settlers near Chester, Pennsylvania, 1682 : together with some account of the English ancestry of the family, including the results of researches by Henry Fishwick, etc. Page 340 [2]
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DNA Connections
It may be possible to confirm family relationships with Jonathan by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Jonathan: