I was surprised to find Charlotte Freeman is the niece of John Eather WILLIAMS, and John Eather WILLIAMS is the uncle of Charlotte Freeman. Their common ancestor is Robert Williams (Williams-73936), son of the first fleeter.
Surname recorded by the church at burial is DEVLON.
2 birth registrations implies twins girls were born.
Sources
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Birth entry
New South Wales Registry of Births, Deaths & Marriage online index, Name: FREEMAN Charlotte and Charlotta, Registration number: 239/1843 V1843239 158, and 923/1843 V1843923 27A, Parents: William and Mary A,
MARRIAGE:
NSW BDM Reg. 3222/1862 ;
Record set: New South Wales Marriages 1788-1945; Subcategory: Civil Marriage & Divorce; Category: Birth, Marriage & Death (Parish Registers); Collections from: Australia & New Zealand; http://search.findmypast.com/record?id=ANZ/BMD/NSW/M/0004755129
DEATH:
NSW BDM Reg. 14830/1923 7596
ALSO:
"Australia, Sydney Branch Genealogical Library, Cemetery Inscriptions, 1800-1960", database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:FLN6-24H : 17 February 2021), Charlotte Devlon in entry for Martha Maude Devlon, 1948.
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DNA Connections
It may be possible to confirm family relationships with Charlotte by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Charlotte: