James Gassaway was born 1734, at All Hallows Parish, Anne Arundel, Maryland, the son of Nicholas and Elizabeth (Hawkins) Gassaway. James was twice married. He and his first wife, Rachel (?nee) were the parents of six sons: James, John, Nicholas, Calab, William and Henry.
James moved to Frederick county, Virginia from Maryland between 1757-1763. In 1777, in Frederick county, Virginia, James married his second wife, Elizabeth Scruggs. They became the parents of seven more children: Thomas, Hannah, Benjamin, Lucy, Rebecca, Samuel and Daniel. 
James died testate. His will dated 14 February 1804, and proved 26 March 1804 in Anderson County, South Carolina, he named his sons Thomas, Benjamin, Samuel and Daniel and his daughters Hannah, Lucy and Rebecca.
He willed his Plantation of 305 acres to his wife Elizabeth. 
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with James: